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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...

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Related Experiment Video

Updated: Jun 29, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

[RNA interference and its promising future].

Li-Sheng Zhang1, Da-Yuan Chen

  • 1State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China. zhanglisheng98@yahoo.com.cn

Yi Chuan = Hereditas
|January 11, 2005
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) uses small interfering RNAs (siRNA) to silence gene expression post-transcriptionally. RNA-dependent RNA polymerase (RdRP) activity may amplify this process, offering future therapeutic applications.

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Last Updated: Jun 29, 2026

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Context:

  • RNA interference (RNAi) is a natural biological process for sequence-specific gene silencing.
  • Small interfering RNAs (siRNA) are key mediators, generated by Dicer from double-stranded RNA (dsRNA).
  • Recent research highlights the role of RNA-dependent RNA polymerase (RdRP) in amplifying RNAi.

Purpose:

  • To review the mechanisms of RNA interference (RNAi).
  • To explore the potential role of RNA-dependent RNA polymerase (RdRP) in amplifying RNAi.
  • To discuss the future applications of RNAi in gene function studies and therapeutics.

Summary:

  • RNA interference (RNAi) mediates post-transcriptional gene silencing via small interfering RNAs (siRNA).
  • RNA-dependent RNA polymerase (RdRP) activity can amplify RNAi by replicating dsRNA triggers or copying siRNAs.
  • This RdRP-mediated amplification converts target mRNA into dsRNA, creating more substrates for Dicer and propagating the silencing effect.

Impact:

  • Understanding RNAi mechanisms, including RdRP amplification, is crucial for its biotechnological applications.
  • RNAi holds significant promise as a tool for functional genomics research.
  • RNAi-based therapeutics offer a potential avenue for targeted gene-specific treatments.