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[The construction of yak liver cDNA library]

Dong-Chun Liang1, Jun Zhang, Juan Xie

  • 1Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China. liangdongchun1225@263.net

Yi Chuan = Hereditas
|January 11, 2005
PubMed

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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