[Analysis of TNFbeta gene microsatellite polymorphism in Graves disease]

The work is to study the microsatellite polymorphism of tumor necrosis factor betagene(TNFc)in Graves disease. The TNFc microsatellite polymorphism in the first intron of tumor necrosis factor betagene was amplified by polymerase chain reaction. The allele frequency and genotype frequency of all samples were analyzed by polyacrylamide gel electrophoresis. The results showed that TNFc microsatellite polymorphism included two alleles and three kinds of genotype. The TNFc2 allele frequency in Graves disease group was greater than control group (chi2 = 4.02,P<0.05), indicating this allele may have an important role in the pathogenesis of Graves disease.