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Related Experiment Videos

[Analysis and mapping of a rice repeated sequence].

Li Jiang1, Xian-Wu Zheng, Xiao-Hong Zhang

  • 1Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101,China. lijiang1029@hotmail.com

Yi Chuan = Hereditas
|January 11, 2005
PubMed
Summary

Researchers identified a novel rice repeat sequence, DH17, crucial for understanding plant genome evolution. This sequence, homologous to known repeats, shows copy number variation between indica and japonica rice varieties and is located on chromosome 12.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Plant Science

Context:

  • Novel repeated DNA sequences play critical roles in genome evolution and function.
  • Nucleotide Binding Site-Leucine Rich Repeat (NBS-LRR) genes are important in plant immunity.
  • Understanding repetitive elements in rice (Oryza sativa) is key to crop improvement.

Purpose:

  • To characterize a newly identified repeated DNA sequence in rice.
  • To investigate the homology and genomic distribution of this repeat.
  • To map the location of the repeat within the rice genome.

Summary:

  • A 560 bp repeated sequence, designated DH17, was isolated from rice NBS-LRR gene amplification.
  • Sequence analysis revealed a 352 bp repeat unit with high homology to OS48 and TrsA, belonging to the same repeat family.

Related Experiment Videos

  • Southern hybridization indicated higher DH17 copy numbers in the indica rice variety (ZYQ8) compared to the japonica variety (JX17).
  • RFLP analysis mapped the tandemly repeated DH17 sequence to the distal end of the long arm of chromosome 12 in a double haploid population.

    • Impact:

    • Provides a new molecular marker for rice genetic studies.
    • Contributes to the understanding of repetitive DNA elements in plant genomes.
    • Highlights potential genetic differences between rice subspecies related to repetitive sequences.