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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
[Gene expression in rice inflorescence development was monitored by using oligonucleotide microarray]
Yuan-Ya Li1, Yun-Sun Zhang, Juan Du
1Department of Biology, School of Life Sciences, Yunnan University, Kunming 650091, China. YYLi199149@yahoo.com.cn
Abstract:
In this paper we chose 50 rice inflorescence genes from Internet,references. Rice oligonucleotide microarray was prepared by printing the target rice inflorescence genes oligonucleotide. Expression patterns of 50 genes from rice inflorescence in three different development phase were obtained by scanning using ScanArray3000 after array hybridization. The scatter plots and scale maps of the images were acquired after the acquired gene expression patterns were analyzed by ImaGene4.0 software. The scatter plots and scale maps show that there existed a significant difference in the expression of these candidate genes in rice inflorescences with different development phase. Further analysis of those candidate gene expression patterns will be helpful to understand the developmental mechanism of rice inflorescence.
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