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Updated: Jul 31, 2026
Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
Published on: February 9, 2010
[Preparation and specificity analysis of anti-Drosophila Dxl6 antibody]
Shan-Zhi Wang1, Liu-Di Yuan, Yong-Qi Wan
1Genetic Research Center, Department of Genetics and Developmental Biology, Southeast University, Nanjing, Jiangsu 210 009, China. wsz.seu@163.net
Abstract:
In order to study the function of Dxl6 which is a novel member of SR protein family, its cDNA was cloned by RT-PCR, and the sequences of its RS domain and its middle part were subcloned into two fusion express vectors, pGEX-4T-1His(6)C and pET32a. After expressing in E.coli BL21, the truncated proteins of Dxl6 RS domain part and Dxl6 middle part in pGEX-4T-1His(6)C were purified and used to immunize rabbits. Purified antibodies against the RS domain and Dxl6 middle part were obtained by affinity chromatography with the expressed products of Dxl6 RS domain part and Dxl6 middle part in pET32a, respectively. The result shows the antibody against Dxl6 RS domain has a good specificity to Dxl6 in Drosophila larvae by Western blot analysis.
