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Updated: Jun 3, 2026

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Published on: November 10, 2009
[An efficient macro-method of genomic DNA isolation from Actinidia chinensis leaves]
Kun-Song Chen1, Fang Li, Chang-Jie Xu
1Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Huajiachi Campus, Hangzhou 310 029, China. akun@zju.edu.cn
Abstract:
It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharose, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.
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