Quantitative real-time reverse transcription-PCR analysis of deformed wing virus infection in the honeybee (Apis mellifera L.)
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Published on: February 8, 2020
Y P Chen1, J A Higgins, M F Feldlaufer
1Bee Research Laboratory, USDA-ARS, Bldg. 476, BARC-East, Beltsville, MD 20705, USA. chenj@ba.ars.usda.gov
Abstract:
Deformed wing virus (DWV) can cause wing deformity and premature death in adult honeybees, although like many other bee viruses, DWV generally persists as a latent infection with no apparent symptoms. Using reverse transcription (RT)-PCR and Southern hybridization, we detected DWV in all life stages of honeybees, including adults with and without deformed wings. We also found DWV in the parasitic mite Varroa destructor, suggesting that this mite may be involved in the transmission of DWV. However, the detection of the virus in life stages not normally associated with mite parasitism (i.e., eggs and larvae) suggests that there are other modes of transmission. The levels of DWV in different life stages of bees were investigated by using TaqMan real-time quantitative RT-PCR. The amounts of virus varied significantly in these different stages, and the highest levels occurred in pupae and in adult worker bees with deformed wings. The variability in virus titer may reflect the different abilities of bees to resist DWV infection and replication. The epidemiology of DWV is discussed, and factors such as mite infestation, malnutrition, and climate are also considered.
Insights
Deformed wing virus (DWV) infects all honeybee life stages and is transmitted by the Varroa destructor mite. Virus levels are highest in pupae and symptomatic adults, indicating varied bee resistance.
Area of Science:
- Apiculture
- Virology
- Insect Pathology
Background:
- Deformed wing virus (DWV) causes significant honeybee losses.
- DWV often establishes latent infections without visible symptoms.
Purpose of the Study:
- To investigate DWV presence and levels across all honeybee life stages.
- To explore potential DWV transmission routes, including the role of Varroa destructor mites.
Main Methods:
- Reverse transcription (RT)-PCR and Southern hybridization for DWV detection.
- TaqMan real-time quantitative RT-PCR to quantify viral loads.
Main Results:
- DWV was detected in all honeybee life stages and in Varroa destructor mites.
- Highest DWV levels were observed in pupae and adult bees exhibiting deformed wings.
- Significant variation in DWV titers suggests differing bee resistance to infection.
Conclusions:
- DWV has multiple transmission pathways beyond mite parasitism.
- Bee resistance varies, influencing DWV replication and disease severity.
- Factors like mite infestation, malnutrition, and climate impact DWV epidemiology.
