Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Non-cross-linking gold nanoparticle aggregation as a detection method for single-base substitutions.

Kae Sato1, Kazuo Hosokawa, Mizuo Maeda

  • 1Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research) Hirosawa 2-1, Wako, Saitama 351-0198, Japan.

Nucleic Acids Research
|January 11, 2005
PubMed
Summary

Gold nanoparticles detect single-base DNA substitutions with high accuracy. This method offers a simple, equipment-free approach for point-of-care diagnosis of genetic variations like single-nucleotide polymorphisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bridging the translational gap in radiotherapy: a human three-dimensional cell culture for evaluating neutron biological effects.

Scientific reports·2026
Same author

Copying a soft lithography master mold using an inexpensive, hobby-use UV-curable resin.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Nucleic acid detection based on single-cluster analysis of cross-linking aggregates of DNA-modified gold nanoparticles using a dark-field microscope.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Development of surface-functionalized power-free microchip for breast cancer cell-derived extracellular vesicle detection.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025
Same author

Protocol for generation of transmitochondrial cybrids under pyruvate/uridine-supplemented conditions using a microfluidic device.

STAR protocols·2025
Same author

Density and structure of DNA immobilised on gold nanoparticles affect sensitivity in nucleic acid detection.

Scientific reports·2025

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Single-base substitutions are crucial in genetic diseases and diagnostics.
  • Existing methods for detecting single-base substitutions often require specialized equipment and complex procedures.
  • Developing accessible and selective detection methods is essential for widespread application.

Purpose of the Study:

  • To demonstrate the utility of DNA-modified gold nanoparticles for detecting single-base substitutions.
  • To leverage the selective aggregation behavior of nanoparticles for mismatch discrimination.
  • To establish a simplified method for analyzing genomic DNA variations.

Main Methods:

  • Utilizing DNA-modified gold nanoparticles that aggregate in a non-cross-linking configuration.

Related Experiment Videos

  • Employing standard molecular biology techniques: DNA extraction, PCR amplification, and single-base primer extension.
  • Observing nanoparticle response (aggregation or dispersion) to primer extension products.

    • Main Results:

    • DNA-modified gold nanoparticles exhibited high selectivity, distinguishing between full matches and terminal mismatches.
    • The nanoparticle assay correctly identified single-base substitutions in unpurified primer extension products.
    • The method was successfully applied to genomic DNA from human tumor cell lines and validated by mass spectrometry and direct sequencing.

    Conclusions:

    • Non-cross-linking aggregation of DNA-modified gold nanoparticles provides extraordinary selectivity for single-base mismatch detection.
    • This technique offers a simplified, equipment-free alternative for single-base substitution analysis.
    • The method holds promise for point-of-care diagnosis of single-nucleotide polymorphisms and other genetic variations.