Related Experiment Video
Updated: Aug 19, 2026
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
[Coxsackie virus B types were discriminated by RT-PCR]
Zhen-yong Li1, Zhi-tao Li, Yan-ming Feng
1Laboratory of Chemobiology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China. zhenyongl@sabc.com.cn
Objective:
To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.
Methods:
A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.
Results:
This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR.
Conclusion:
The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.
More Related Videos
07:40Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
Published on: June 29, 2020
03:53Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
Published on: November 10, 2023
