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Published on: July 11, 2014
Stereoselective high-performance liquid chromatographic determination of flurbiprofen in human plasma
G Geisslinger1, S Menzel-Soglowek, O Schuster
1Department of Pharmacology and Toxicology, University of Erlangen-Nürnberg, Germany.
Abstract:
An enantioselective high-performance liquid chromatographic assay for the quantitation of the enantiomers of flurbiprofen in human plasma is described. The procedure involved extraction of flurbiprofen from acidified plasma into hexane-diethyl ether (8:2, v/v). Stereoselective separation was achieved with a prepacked alpha 1-acid glycoprotein column without any derivatization procedure. A second assay using a conventional reversed-phase column to determine racemic flurbiprofen is also described. The detection wavelength was set at 246 nm. The limit of quantification was found to be 50 ng/ml for both assays. The method was demonstrated to be sufficiently sensitive for stereoselective pharmacokinetic studies of flurbiprofen.
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