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HCO(3)(-)-independent rescue from apoptosis by stilbene derivatives in rat cardiomyocytes
Shigeru Tanabe1, Xiaoming Wang, Nobuyuki Takahashi
1Department of Cell Physiology and Molecular Physiology, National Institute for Physiological Sciences, Japan Science and Technology Agency, Myodaiji-cho, Okazaki 444-8585, Japan.
Abstract:
Apoptosis of rat cardiomyocytes induced by staurosporine is prevented by a stilbene derivative (DIDS), which is a known blocker of both Cl(-)/HCO(3)(-) exchangers and Cl(-) channels. To clarify its target, staurosporine-induced activation of caspase-3, DNA laddering and cell death were examined in cultured rat cardiomyocytes. Removal of ambient HCO(3)(-), which minimizes the function of Cl(-)/HCO(3)(-) exchangers, failed to affect the preventive effect of DIDS on apoptosis. A carboxylate analog Cl(-) channel blocker, which does not block Cl(-)/HCO(3)(-) exchangers, also inhibited apoptotic events. Thus, rescue by DIDS of cardiomyocytes from apoptosis is mediated by blockage of Cl(-) channels.
