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Related Experiment Videos

The offloading model for dynein function: differential function of motor subunits.

Wei-Lih Lee1, Michelle A Kaiser, John A Cooper

  • 1Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

The Journal of Cell Biology
|January 12, 2005
PubMed
Summary
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Dynein

Area of Science:

  • Cell Biology
  • Molecular Motors
  • Cytoskeletal Dynamics

Background:

  • Dynein is crucial for mitotic spindle positioning in budding yeast.
  • An offloading model proposes dynein targets microtubule plus ends, detaches to the cortex, and pulls.
  • The roles of dynein's accessory chains, intermediate chain (IC) and light intermediate chain (LIC), were unclear.

Purpose of the Study:

  • To investigate the functional roles of dynein intermediate chain (IC) and light intermediate chain (LIC) in budding yeast mitosis.
  • To elucidate the specific contributions of IC and LIC to dynein targeting and cortical offloading.

Main Methods:

  • Functional studies of dynein IC (Pac11) and LIC (Dyn3) in budding yeast.
  • Analysis of dynein component localization to microtubule plus ends and the cell cortex.

Related Experiment Videos

  • Investigating the dependencies between dynein heavy chain (HC/Dyn1), IC, and LIC localization.

    • Main Results:

    • Both IC and LIC are essential for dynein function, similar to HC.
    • IC and LIC, like HC, target microtubule plus ends, dependent on HC.
    • HC plus-end targeting requires IC but not LIC.
    • IC and HC localize to cortical dots (offloading sites), but LIC does not.
    • Cortical dot localization of HC requires both IC and LIC.

    Conclusions:

    • Dynein intermediate chain (IC) and light intermediate chain (LIC) have distinct, essential roles in dynein-mediated spindle positioning.
    • IC is required for HC targeting to microtubule plus ends, while both IC and LIC are necessary for HC cortical offloading and activation.
    • These findings provide critical insights into the proposed dynein offloading model.