[Pseudogermin activity during maturation and germination of wheat seeds]

The OxO activities of psiG during maturation and germination of wheat seeds were measured by SDS-PAGE followed by OxO activity staining. The results showed that there was no expression of G and G', but only that of psiG during wheat seeds maturation (Fig. 2). 10 days after anthesis, the OxO activity of psiG was observed in glumes, palea, lemma, seed coat and pericarp, then the OxO activities in glumes, lemma, seed coat and pericarp increased rapidly and remained high until the seeds matured (Fig. 2). Basing on the observation that psiG was expressed mainly in the tissues abundant in lignin when cell growth just stopped, it was suggested that psiG may function in promoting lignification of cell wall of glumes, palea, lemma, seed coat and pericarp by producing H(2)O(2) by oxidation oxalate. During wheat seeds germination, psiG was present in vascular transition region in addition to G and G' in Zhongyu 5 (Fig. 3), but it was not necessary for germination.