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Development of a bioactive packaging cellophane using Nisaplin as biopreservative agent
N P Guerra1, C L Macías, A T Agrasar
1Departamento de Bioquímica, Xenética e Inmunoloxía, Facultade de Ciencias de Ourense, Universidade de Vigo. As Lagoas, Ourense, Spain.
Letters in Applied Microbiology
|January 13, 2005
Summary
This study developed a nisin-coated cellophane for meat packaging. The bioactive packaging significantly reduced bacteria in chopped meat, extending shelf life under refrigeration.
Area of Science:
- Food Science
- Microbiology
- Materials Science
Background:
- Controlling microbial growth in processed meats is crucial for food safety and shelf-life extension.
- Nisin, a bacteriocin, exhibits potent antimicrobial activity against Gram-positive bacteria.
- Cellophane is a biodegradable polymer with potential for active packaging applications.
Purpose of the Study:
- To develop a nisin-containing cellophane-based coating for chopped meat packaging.
- To evaluate the antimicrobial efficacy of the developed bioactive packaging.
- To assess the impact on microbial load and shelf life of fresh veal meat.
Main Methods:
- Nisin adsorption onto cellophane 'P' type surfaces was investigated at various temperatures (8-60°C) and concentrations.
- Antimicrobial activity was tested by incorporating the nisin-adsorbed cellophane into fresh veal meat.
- Total aerobic bacteria counts were monitored over 12 days of storage at 4°C.
Main Results:
- Nisin adsorption to cellophane was optimal at 8°C.
- The developed bioactive cellophane significantly reduced total aerobic bacteria in veal meat by approximately 1.5 log units.
- This reduction was observed throughout 12 days of refrigerated storage.
Conclusions:
- Bioactive cellophane packaging incorporating nisin is effective in controlling microbial growth in chopped meat.
- This technology offers a promising strategy for extending the shelf life of refrigerated chopped meat.