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Simple, robust strategies for generating DNA-directed RNA interference constructs.

Robert R Rice1, Andrew N Muirhead, Bruce T Harrison

  • 1Benitec Ltd., University of Queensland, St. Lucia QLD, Australia.

Methods in Enzymology
|January 13, 2005
PubMed
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Two novel methods, oligonucleotide assembly (OA) and long-range cloning (LRC), simplify the creation of DNA-directed RNA interference (ddRNAi) constructs for expressing hairpin RNA (hpRNA). These techniques offer efficient solutions for generating complex gene expression vectors.

Failed At:

2026-06-19T13:35:47.642292+00:00

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