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Updated: Apr 7, 2026
Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
Published on: April 28, 2015
Synthesis, characterization and immobilization of Prussian blue nanoparticles. A potential tool for biosensing
Pablo A Fiorito1, Vinicius R Gonçales, Eduardo A Ponzio
1Instituto de Quimica, Universidade de Sao Paulo, C.P. 26077, 05513-970, Sao Paulo (SP), Brazil.
Abstract:
Prussian blue (PB) particles with the size of ca. 5 nm were synthesized and immobilized in a multilayer structure, as a strategy for the potential development of an amperometric transducer for oxidase-enzyme-based biosensors. Multilayer films composed of PB and poly(allylamine hydrochloride) (PAH) were prepared via layer-by-layer (LbL) sequential deposition. The process was carefully monitored by UV-vis spectroscopy and cyclic voltammetry. The increase of the redox current peaks during the layer-by-layer deposition demonstrated that charge propagation within the film occurs. Linear increase of UV-vis absorbance with the number of deposited bilayers indicates that well-organized systems have been elaborated. ITO electrodes coated with PB/PAH films were used successfully for detecting H2O2, sensitivity being dependent on the number of PB/PAH layers.
