Related Experiment Video
Updated: Aug 15, 2026

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
Published on: August 15, 2011
Interaction between a nanovirus-like component and the Tobacco curly shoot virus/satellite complex
1Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
Abstract:
The biological role of DNA1, a nanovirus-like component shown to be associated with the begomovirus/satellite complex, has not yet been identified. Here, we demonstrated that DNA1 of Tobacco curly shoot virus isolate Y35 (TbCSV-Y35) attenuated leaf-curling symptoms induced by TbCSV-Y35 or TbCSV-Y35 plus Y35 DNAbeta in the early stage of symptom development and induced leaf cluster at a later stage of symptom development in Nicotiana benthamiana plants. The leaf disc assay demonstrated that TbCSV-Y35 DNA1 replicated autonomously. Southern blot analysis revealed that TbCSV-Y35 DNA1 reduced viral DNA accumulation. Viral DNA accumulation was not reduced when plants were co-inoculated with TbCSV-Y35 DNAbeta, but the TbCSV-Y35 DNAbeta level was dramatically reduced in the presence of TbCSV-Y35 DNA1. To determine whether the interaction between TbCSV/satellite complex and DNA1 had isolate specificity, DNA1 of TbCSV isolate Y132 was cloned and sequenced. It was found to have 75% nucleotide sequence identity with TbCSV-Y35 DNA1. Infectivity tests showed that TbCSV-Y132 DNA1 had no effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAbeta in N. benthamiana plants, although Y132 DNA1 could replicate in these plants.
More Related Videos
08:14Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Published on: February 14, 2013
23:21Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
Published on: April 19, 2014
Related Concept Videos
Viral Structure
Protein Complex Assembly
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Introduction to Virus
Subviral Agents
Inhibitors Of Virion Release
Inhibitors of Virion Maturation and Assembly