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Gene expression profile analysis of mouse colon embryonic development.
Young-Kyu Park1, Jeffrey L Franklin, Stephen H Settle
1Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Genesis (New York, N.Y. : 2000)
|January 13, 2005
Summary
Researchers profiled gene expression in the developing mouse colon (embryonic day 13.5-18.5) to understand genetic programs. They identified distinct gene expression patterns linked to epithelial and mesenchymal maturation during colonic development.
Area of Science:
- Developmental Biology
- Genomics
- Gastroenterology
Background:
- The mouse colon undergoes significant morphological changes during late embryogenesis.
- Development involves the transition from pseudostratified endoderm to a single-layered columnar epithelium.
- Mesenchymal maturation accompanies epithelial changes.
Purpose of the Study:
- To identify regulatory genetic programs governing mouse colon development.
- To profile gene expression changes during late embryogenesis (embryonic day 13.5 to 18.5).
- To create a molecular portrait of colonic development.
Main Methods:
- Gene expression profiling using microarrays.
- Analysis of 13,484 complementary DNA (cDNA) elements.
- Unbiased cluster analysis to identify gene expression patterns.
Main Results:
- Two distinct gene expression groups correlated with epithelial and mesenchymal maturation.
- 158 upregulated genes are associated with colonic differentiation.
- 49 downregulated genes are linked to nuclear organization, transcriptional regulation, and cell proliferation.
Conclusions:
- The study provides a molecular atlas of mouse colon development.
- Identified genes offer insights into differentiation, proliferation, and organization.
- This resource aids research in colonic development, neoplasia, and comparative organogenesis.