Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Systematic search for natural antisense transcripts in eukaryotes (review).

Oystein Røsok1, Mouldy Sioud

  • 1Institute for Cancer Research, Molecular Medicine Group, Montebello, N-0310 Oslo, Norway.

International Journal of Molecular Medicine
|January 14, 2005
PubMed
Summary

Bioinformatic tools predict many natural antisense transcripts, but experimental validation remains limited. A new experimental method enables systematic identification of these crucial RNA pairs in any cell or tissue type.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Immunity: Current Progress and Future Directions of CAR-T Cell Therapy.

International journal of molecular sciences·2026
Same author

RNA Therapeutics: Bridging Discovery and Clinical Implementation.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Molecular Mechanisms of Innate Immune Sensing of Exogenous RNAs.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Effects of Nucleoside Modifications on mRNA Translation: Choosing the Right Modifications.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Assessing the Immunogenicity of Synthetic RNA Using Blood Cells.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Large-Scale Production of Unmodified mRNA for Reprogramming Human Dendritic Cells and T Cells.

Methods in molecular biology (Clifton, N.J.)·2025

Area of Science:

  • Genomics
  • Transcriptomics
  • Bioinformatics

Background:

  • The advent of complete genome sequences has enabled large-scale prediction of natural antisense transcripts (NATs) using bioinformatics.
  • Over 1,600 human and 2,500 murine NAT pairs have been predicted computationally.
  • Current bioinformatic approaches face limitations in experimental validation and tissue-specific identification of NATs.

Purpose of the Study:

  • To address the limitations of bioinformatic prediction of natural antisense transcripts.
  • To develop and present an experimental strategy for systematic identification of sense-antisense transcript pairs.

Main Methods:

  • Development of an experimental approach based on double-stranded cDNA selection.
  • Utilizing hybridization between first-strand cDNAs generated in the presence of actinomycin D.

Related Experiment Videos

  • This method facilitates the systematic analysis of sense and antisense transcript partners.

    • Main Results:

    • A novel experimental strategy has been established for identifying sense-antisense transcript pairs.
    • This approach overcomes limitations of computational methods for NAT discovery.
    • Enables systematic analysis across diverse cell and tissue types.

    Conclusions:

    • The new experimental strategy provides a robust method for identifying natural antisense transcripts.
    • This advancement is crucial for the experimental validation of computationally predicted NATs.
    • Facilitates comprehensive studies of NATs in specific biological contexts.