Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An in situ toxicity identification evaluation method Part II: Field validation.

G Allen Burton1, Juanita F Nordstrom

  • 1Institute for Environmental Quality, Wright State University, Dayton, Ohio 45435, USA. allen.burton@wright.edu

Environmental Toxicology and Chemistry
|January 15, 2005
PubMed
Summary

An in situ toxicity identification evaluation (iTIE) chamber offers a more sensitive method for assessing sediment toxicity compared to traditional lab methods. This approach accurately identifies causative toxic chemical fractions in pore water.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unintentional Disinformation: A Cautionary Tale for Scientific Publishing.

Environmental toxicology and chemistry·2026
Same author

Distinguishing Natural and Anthropogenic stressors at a Groundwater-Surface Water Interface Using a Weight-of-Evidence Framework.

Integrated environmental assessment and management·2026
Same author

A Plea for Cumulative Stressor Risk Assessments in Light of Climate Change.

Environmental science & technology·2025
Same author

Teasing out the Effects of Natural Stressors at Chemically Contaminated Sites.

Environmental toxicology and chemistry·2024
Same author

Has the Rio Doce "time bomb" been defused? Using a weight-of-evidence approach to determine sediment quality.

Integrated environmental assessment and management·2023
Same author

Evaluation of Capping Materials to Reduce Zinc Flux from Sediments in a Former Mining Pit Lake.

Environmental toxicology and chemistry·2021

Area of Science:

  • Environmental Chemistry
  • Ecotoxicology
  • Aquatic Toxicology

Background:

  • Sediment toxicity often results from complex chemical mixtures, making it challenging to pinpoint causative agents.
  • Traditional laboratory-based Toxicity Identification Evaluation (TIE) methods can alter chemical bioavailability and introduce artifacts due to sample collection and manipulation.

Purpose of the Study:

  • To introduce and evaluate an in situ Toxicity Identification Evaluation (iTIE) chamber for fractionating and assessing pore-water toxicity in sediments.
  • To compare the effectiveness and sensitivity of the iTIE method against the U.S. Environmental Protection Agency (U.S. EPA) TIE method.

Main Methods:

  • An iTIE chamber was deployed directly into sediments to fractionate pore-water chemicals into nonpolar, metal, and ammonia-type groups.

Related Experiment Videos

  • Field studies were conducted at three contaminated sites (PAHs, PCBs, chlorobenzenes).
  • Toxicity was assessed using 24-h exposures with Daphnia magna for both iTIE and U.S. EPA TIE methods.

    • Main Results:

    • The iTIE method demonstrated significantly higher toxicity detection compared to the laboratory TIE method.
    • iTIE successfully separated dominant chemical classes, identifying fractions contributing to toxicity.
    • The laboratory TIE method resulted in a loss of toxicity, hindering subsequent stressor identification.

    Conclusions:

    • The in situ Toxicity Identification Evaluation (iTIE) method provides a more accurate and sensitive evaluation of pore water toxicity than laboratory TIE.
    • iTIE offers advantages including reduced artifacts, realistic in situ exposure, and applicability to diverse substrates.
    • Limitations include pretest assembly, fewer fractionation options, and shallow water restrictions.