Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput mass spectrometer using atmospheric pressure ionization and a cylindrical ion trap array.

Alexander S Misharin1, Brian C Laughlin, Andrey Vilkov

  • 1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.

Analytical Chemistry
|January 15, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serine Octamer Substitution Reactions With α-Hydroxy Acids.

Rapid communications in mass spectrometry : RCM·2026
Same author

Molecular Framework Modification in Mass Spectrometry: Atom Exchange, Insertion, and Deletion.

Journal of the American Society for Mass Spectrometry·2026
Same author

Early-stage drug discovery in a new-generation ultrahigh-throughput mass spectrometry platform.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

High-Throughput Reactive Desorption Electrospray Ionization Mass Spectrometry for Targeted Derivatization and Analysis of Poorly Ionized Small Molecules.

Analytical chemistry·2026
Same author

High-Throughput Small-Scale Platform for Synthesis, Characterization, and Modeling of Per- and Polyfluoroalkyl Substances Analogs.

Environmental science & technology letters·2026
Same author

Accelerated Synthesis of Pyrazoles Mediated by Water Microdroplets.

ACS sustainable chemistry & engineering·2026

This study evaluates a new high-throughput mass spectrometer with multiple channels for simultaneous sample analysis. Initial results show promise for applications in proteomics and metabolomics, despite some limitations in dynamic range and mass discrimination.

Area of Science:

  • Analytical Chemistry
  • Instrumentation Science

Background:

  • High-throughput analysis is crucial for fields like proteomics and metabolomics.
  • Existing mass spectrometry techniques can be limited by throughput and multiplexing capabilities.

Purpose of the Study:

  • To investigate the analytical performance of a novel multi-channel, high-throughput mass spectrometer.
  • To assess the feasibility of simultaneous analysis of diverse sample types using multiplexed channels.

Main Methods:

  • Utilized a custom-built mass spectrometer with two fully multiplexed, independent channels based on cylindrical ion traps.
  • Employed a multichannel electrospray ionization source for introducing organic compounds, peptides, and proteins.
  • Evaluated cross-talk, mass-to-charge ratio limits, resolution, dynamic range, and mass discrimination.

Related Experiment Videos

Main Results:

  • Achieved unit mass-to-charge resolution up to approximately 800 Th and an upper mass limit of ~1300 Th.
  • Minimized cross-talk between channels to 1-2% through detector shielding.
  • Demonstrated analysis of alkylamine and peptide mixtures, but noted limitations with wide mass-to-charge ratios due to ion optics.

Conclusions:

  • The multi-channel mass spectrometer shows potential for high-throughput analysis, particularly in proteomics and metabolomics.
  • Further optimization of vacuum systems and ion optics is needed to enhance dynamic range and address mass discrimination for broader applications.