Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Digital microfluidics with in-line sample purification for proteomics analyses with MALDI-MS.

Aaron R Wheeler1, Hyejin Moon, Christopher A Bird

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA.

Analytical Chemistry
|January 15, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A digital microfluidic platform for cell-based non-invasive testing.

iScience·2026
Same author

DROP-LCMS for wastewater surveillance of viral disease.

Lab on a chip·2026
Same author

Potent Bacteriostatic Activity of a Naphthalene-2-Acyl Imidazolium Salt against Xanthomonas oryzae pv. oryzae.

The plant pathology journal·2026
Same author

Understanding the Mechanism of Triplet-Triplet Energy Transfer in the Photocatalytic [2 + 2] Cycloaddition: Insights From Quantum Chemical Modeling.

Journal of computational chemistry·2025
Same author

A combined digital microfluidic test for assessing infection and immunity status for viral disease in saliva.

Lab on a chip·2025
Same author

A compartmentalization-free microfluidic digital assay for detecting picogram levels of protein analytes.

Lab on a chip·2025

A novel electrowetting-on-dielectric (EWOD) method purifies samples for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). This digital microfluidics technique enhances signal-to-noise ratios and improves protein analysis efficiency.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Microfluidics

Background:

  • Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is crucial for proteomics.
  • Sample impurities, such as salts and urea, significantly interfere with MALDI-MS analysis.
  • Current purification methods can be time-consuming and less efficient for complex samples.

Purpose of the Study:

  • To develop and validate an in-line sample purification technique for MALDI-MS using digital microfluidics.
  • To assess the effectiveness of the electrowetting-on-dielectric (EWOD) method in removing various impurities.
  • To evaluate the impact of EWOD-based purification on spectral quality and quantitative analysis.

Main Methods:

  • Utilized electrowetting-on-dielectric (EWOD) for digital microfluidic droplet manipulation.

Related Experiment Videos

  • Developed an in-line workflow involving impurity removal with water and subsequent matrix addition.
  • Applied the method to purify contaminated peptide samples and protein digests.

    • Main Results:

    • Successfully reduced salt adduct peaks and mitigated the effects of high impurity concentrations (e.g., urea).
    • Demonstrated an increased signal-to-noise (S/N) ratio in MALDI-MS spectra with purified samples.
    • Showed improved efficiency compared to conventional reversed-phase-pipet tip purification for protein digests.

    Conclusions:

    • The developed EWOD-based in-line purification is an effective method for enhancing MALDI-MS analysis.
    • This technique offers a robust solution for handling challenging samples in proteomics.
    • Anticipated to facilitate the development of integrated, high-throughput proteomics analysis platforms.