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Investigation of oxidative stress during fracture healing in the rats
Hasan Yeler1, Filiz Tahtabas, Ferda Candan
1Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Cumhuriyet, 58140, Sivas, Turkey.
Abstract:
One of the most damaging effects of reactive oxygen species (ROS) is lipid peroxidation, the end-product of which is malondialdehyde (MDA). This study was aimed to evaluate erythrocyte MDA levels during fracture healing in rats. Thirty male rats were used and the rats were divided into two groups to serve as controls and tests. Six rats were used as a control group that was not subject to fracture. The remaining 24 rats were divided into four groups and erythrocyte MDA levels were examined on days 5, 10, 20 and 30 post fracture. The right fibulas of rats were broken by manual angulation in the experimental group. The erythrocyte malondialdehyde level was measured in the experimental and control groups. The difference between malondialdehyde levels of control and experimental groups was statistically significant (p<0.05). Oxidative stress clearly increases during fracture healing in rats.
Insights
Fracture healing in rats increases oxidative stress, indicated by higher malondialdehyde (MDA) levels in red blood cells. This study measured MDA to assess lipid peroxidation during bone repair.
Area of Science:
- Biomedical Science
- Physiology
- Toxicology
Background:
- Reactive oxygen species (ROS) cause significant cellular damage.
- Lipid peroxidation, a key indicator of oxidative stress, results in malondialdehyde (MDA) formation.
Purpose of the Study:
- To investigate erythrocyte malondialdehyde (MDA) levels during the fracture healing process in a rat model.
- To determine the correlation between fracture healing and oxidative stress markers.
Main Methods:
- Thirty male rats were divided into control (no fracture) and experimental (fractured fibula) groups.
- Erythrocyte MDA levels were measured on days 5, 10, 20, and 30 post-fracture.
- Statistical analysis (p<0.05) was used to compare MDA levels between groups.
Main Results:
- A statistically significant increase in erythrocyte MDA levels was observed in the fracture group compared to the control group (p<0.05).
- MDA levels indicated elevated lipid peroxidation during the fracture healing period.
Conclusions:
- Fracture healing in rats is associated with a significant increase in oxidative stress.
- Erythrocyte MDA levels serve as a reliable biomarker for monitoring oxidative stress during bone repair.
