Related Experiment Videos
Lectin KM+-induced neutrophil haptotaxis involves binding to laminin
Luciane Ganiko1, Antônio R Martins, Edna Freymüller
1Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, 14049-900, Ribeirão Preto, SP, Brazil.
Biochimica Et Biophysica Acta
|January 18, 2005
Summary
The lectin KM+ (artocarpin) binds to laminin, enhancing its ability to guide neutrophil migration. This interaction is crucial for forming the necessary gradient for neutrophil haptotaxis.
Area of Science:
- Immunology
- Glycobiology
- Cell Biology
Background:
- The lectin KM+ (artocarpin) from Artocarpus integrifolia induces neutrophil migration via haptotaxis.
- KM+ interaction with extracellular matrix and neutrophils relies on recognizing mannose-containing glycans.
Purpose of the Study:
- To investigate the binding of KM+ to laminin.
- To determine if KM+-laminin interaction potentiates KM+-induced neutrophil migration.
Main Methods:
- Tissue labeling with KM+ and analysis of ligand localization in lung tissue.
- Inhibition studies using D-mannose and specific mannosyl oligosaccharides.
- Co-localization studies using KM+ and anti-laminin antibodies on lung extracts and tissue.
- Neutrophil migration assays using soluble KM+ with and without laminin-coated substrates.
Main Results:
- KM+ binds to laminin, a component of the basement membrane and interstitial connective tissue.
- KM+ ligands in lung tissue are inhibited by mannose and specific mannosyl oligosaccharides.
- KM+ and anti-laminin antibodies recognize similar high molecular mass components and show co-localized labeling in lung basement membranes.
- Laminin coating reversed the inability of low concentrations of soluble KM+ to induce human neutrophil migration.
Conclusions:
- Laminin is a tissue ligand for KM+.
- The KM+-laminin interaction is relevant to KM+-induced neutrophil migration.
- KM+ interaction with laminin promotes substrate-bound KM+ gradient formation, enabling neutrophil haptotaxis.