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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

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Published on: October 9, 2014

Alternative splicing regulation impacts heart development.

Thomas A Cooper1

  • 1Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.

Cell
|January 18, 2005
PubMed
Summary
This summary is machine-generated.

A study shows that removing the ASF/SF2 SR protein in hearts causes cardiomyopathy and disrupts gene splicing during early development. This highlights the critical role of SR proteins in heart function and gene regulation.

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Area of Science:

  • Molecular Biology
  • Gene Expression Regulation
  • Cardiovascular Science

Background:

  • SR proteins are crucial for posttranscriptional gene control, particularly alternative splicing.
  • Alternative splicing allows a single gene to produce multiple protein isoforms, increasing proteomic diversity.

Discussion:

  • Xu et al. (2005) investigated the role of the SR protein ASF/SF2 in heart development.
  • A heart-specific knockout of ASF/SF2 was created to study its function.
  • The study focused on the impact of ASF/SF2 loss on cardiac function and splicing patterns.

Key Insights:

  • Knocking out ASF/SF2 in the heart leads to the development of cardiomyopathy.
  • Loss of ASF/SF2 results in the misregulation of specific alternative splicing events.
  • These splicing defects occur during early postnatal development, indicating a critical temporal role.

Outlook:

  • Understanding ASF/SF2's role can inform therapeutic strategies for heart diseases linked to splicing defects.
  • Further research could explore other SR proteins and their specific contributions to cardiac development and disease.
  • This work underscores the intricate link between splicing regulation and cardiovascular health.