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Updated: Aug 20, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Expression of the type I interferon receptor and the interferon-induced Mx protein in human endometrium during the
Tomoya Ozaki1, Kentaro Takahashi, Haruhiko Kanasaki
1Department of Obstetrics and Gynecology, Seirei Hamamatsu General Hospital, Hamamatsu, Japan.
Objective:
To investigate the expression of the type I interferon receptor (IFNAR) and interferon-induced Mx protein (Mx) in normal human endometrium throughout the menstrual cycle.
Design:
Prospective study.
Setting:
Medical university in Japan.
Patient(S):
Thirty-seven normal endometrial tissues from fertile women who had undergone hysterectomies for reasons other than endometrial disease.
Intervention(S):
IFNAR-1, IFNAR-2, MxA, and MxB gene expression was analyzed by reverse transcription-real-time quantitative polymerase chain reaction. Moreover, localization of IFNAR-1 and IFNAR-2 were studied by immunohistochemistry.
Main Outcome Measure(S):
Expression of IFNAR-1, IFNAR-2, MxA, and MxB.
Result(S):
Expression of IFNAR-2 gene was significantly increased in the menstrual and midsecretory phase as compared with in the proliferative phase. Immunohistochemistry for IFNAR-1 and IFNAR-2 revealed weak staining of glandular epithelium and weak staining of stromal cells during the proliferative phase. However, an intense immunohistochemical staining of IFNAR-2 was observed on the surface and basement membrane of glands in the secretory phase. There was no statistical difference between MxA and MxB gene expression throughout the menstrual cycle.
Conclusion(S):
Our results suggest that IFNAR and Mx are expressed in the human endometrium and that the expression of IFNAR is cyclically changed during the menstrual cycle.
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