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Polyphasic study of wine Lactobacillus strains: taxonomic implications
A M Rodas1, S Ferrer1, I Pardo1
1ENOLAB - Laboratori de Microbiologia Enològica, Departament de Microbiologia i Ecologia, Facultat de Ciències Biològiques, Universitat de València, Dr Moliner 50, 46100 Burjassot, València, Spain.
International Journal of Systematic and Evolutionary Microbiology
|January 18, 2005
Summary
This study characterized 178 wine lactobacilli using classical and molecular methods. Random Amplification of Polymorphic DNA (RAPD) and ribotyping proved effective for both identifying and typing these important wine microorganisms.
Area of Science:
- Microbiology
- Food Science
- Molecular Biology
Background:
- Lactobacilli are crucial for wine fermentation and quality.
- Accurate identification and typing of wine lactobacilli are essential for microbial ecology and quality control.
Purpose of the Study:
- To characterize 178 lactobacilli strains isolated from wine using a polyphasic approach.
- To evaluate the suitability of various phenotypic and molecular techniques for the identification and typing of wine lactobacilli.
- To propose optimized molecular procedures for wine microorganism analysis.
Main Methods:
- Phenotypic identification using classical tests (morphology, fermentation, growth, lactic acid isomer).
- Molecular characterization including Random Amplification of Polymorphic DNA (RAPD), 16S rDNA Restriction Analysis (16S-ARDRA), Pulsed-Field Gel Electrophoresis-RFLP (PFGE-RFLP), and ribotyping.
- Data analysis using BioNumerics software to generate taxonomic dendrograms.
Main Results:
- Phenotypic and molecular data were integrated to analyze strain relationships.
- Random Amplification of Polymorphic DNA (RAPD) and ribotyping were identified as effective for both identification and typing.
- 16S-ARDRA was suitable for identification, while PFGE-RFLP was useful for typing only.
- The wine strains were identified as Lactobacillus brevis, L. collinoides, L. hilgardii, L. paracasei, L. pentosus, L. plantarum, and L. mali.
Conclusions:
- A polyphasic approach provides comprehensive characterization of wine lactobacilli.
- RAPD and ribotyping are recommended for efficient identification and typing of these wine-associated bacteria.
- The study successfully identified and classified multiple Lactobacillus species commonly found in wine environments.