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Related Experiment Videos

Bub1 multitasking in mitosis.

Hongtao Yu1, Zhanyun Tang

  • 1Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA. hongtao.yu@utsouthwestern.edu

Cell Cycle (Georgetown, Tex.)
|January 19, 2005
PubMed
Summary
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The protein kinase Bub1 ensures accurate chromosome inheritance during cell division. Bub1 maintains sister chromatid cohesion and regulates the spindle checkpoint, preventing errors in mitosis.

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Accurate chromosome segregation during mitosis requires sister chromatid cohesion until bipolar spindle attachment.
  • The spindle checkpoint monitors chromosome attachment and delays mitosis if errors are detected.
  • The anaphase-promoting complex/cyclosome (APC/C) controls cell cycle progression, including the initiation of anaphase.

Purpose of the Study:

  • To elucidate the multifaceted roles of the protein kinase Bub1 in ensuring accurate chromosome inheritance during mitosis.
  • To investigate Bub1's functions in both spindle checkpoint regulation and non-checkpoint processes.

Main Methods:

  • Biochemical studies investigating protein interactions and phosphorylation events.
  • Analysis of kinetochore localization of checkpoint proteins.

Related Experiment Videos

  • Examination of centromeric cohesion maintenance mechanisms.

    • Main Results:

    • Bub1 is a key component of the spindle checkpoint, regulating kinetochore localization of Mad2 and other checkpoint proteins.
    • Bub1 directly phosphorylates and inhibits Cdc20, a crucial activator of the APC/C.
    • Bub1 also functions independently of the spindle checkpoint to preserve centromeric cohesion via MEI-S332/shugoshin proteins.

    Conclusions:

    • Bub1 plays critical, diverse roles in mitosis, encompassing both spindle checkpoint control and direct maintenance of chromosome cohesion.
    • These multiple functions of Bub1 are essential for preventing chromosome mis-segregation and ensuring proper inheritance of genetic material.