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Published on: April 20, 2018
Development and mapping of microsatellite (SSR) markers in wheat
1Soybean Genomics and Improvement Lab, Beltsville Agricultural Research Center, USDA-ARS, MD, 20705, USA.
Summary
Researchers developed new microsatellite DNA markers for hexaploid wheat, enhancing genome mapping efficiency. These new markers provide more informative genetic data for wheat breeding and research.
Area of Science:
- Plant Genetics
- Molecular Biology
- Genomics
Background:
- Microsatellite DNA markers are highly informative for hexaploid wheat genetics.
- Existing markers have limitations in wheat genome mapping.
Purpose of the Study:
- Develop novel microsatellite-flanking primers for wheat.
- Map these new loci onto the wheat genome using genetic and physical methods.
Main Methods:
- Developed 540 microsatellite-flanking primer pairs using improved library creation techniques.
- Utilized ITMI W7984 x Opata85 recombinant inbred line population for genetic linkage mapping.
- Employed physical mapping with cytogenetic stocks for locus localization.
Main Results:
- Successfully mapped 347 loci using 315 primer sets, with 125 localized by physical mapping alone.
- Assigned loci to the A, B, and D genomes (126, 125, and 96, respectively).
- Extended the wheat linkage map by 80.7 cM, with high consistency between genetic and physical mapping.
Conclusions:
- Developed efficient methods for wheat microsatellite marker development.
- Enhanced the wheat genome map with new loci, improving marker density and coverage.
- New markers provide valuable tools for wheat genetic studies and crop improvement.
