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Published on: May 17, 2013
Tiam1 gene expression and its significance in colorectal carcinoma
Li Liu1, De-Hua Wu, Yan-Qing Ding
1Department of Pathology, First Military Medical University, Guangzhou 510515, Guangdong Province, China.
Aim:
To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis.
Methods:
Expressions of Tiam1 gene in 8 colorectal carcinoma cell lines were detected by reverse transcriptase-polymerase chain reaction. In vitro invasiveness was determined by means of Matrigel invasion assay. The correlation of Tiam1 expression with the invasive ability was also analyzed.
Results:
Tiam1 gene was highly expressed in LoVo and SW620, which were established from metastatic colorectal carcinomas in comparison with LS174T, SW480, HCT116, LST, HRT-18 and Hee8693, which were established from primary colorectal carcinomas. In vitro cell invasion demonstrated that LoVo and SW620 had a higher invasive ability than LS174T, SW480, HCT116, LST, HRT-18 and Hee8693. The expression of Tiam1 gene was highly related to the metastatic potential of colorectal carcinoma cells.
Conclusion:
Tiam1 gene may play an important role in invasion and metastasis of colorectal carcinoma and is a metastasis-related gene.
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