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[Determination of carvacrol and thymol in Mosla chinensis by HPLC]
Li Ji1, Fang Wang, Yuan-yan Liu
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Objective:
To establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis.
Method:
The sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm.
Result:
The linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%.
Conclusion:
The method is reliable, and can be used for quality control of M. chinensis.