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Published on: February 20, 2012
[Genetic analysis of cultivated ginseng population with the assistance of RAPD technology]
Ai-juan Shao1, Xin Li, Lu-qi Huang
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Objective:
To provide reference for breeding of cultivated ginseng by researching the genetic diversities among different strains.
Method:
To make up the systematic diagram of genetic relationship by calculating the proximity coefficient with SPSS 10.0 software and clustered by between-groups linkage method.
Result:
The selected 18 random primers used in PCR amplification to produce 145 bands in 17 samples, among which 53 bands (36.5%) are polymorphic. The results of cluster analysis show that the genetic relationship between different strains of Da-maya is closer than that of Er-maya, which proves that it is feasible to differentiate the cultivated groups in molecular level on outside character.
Conclusion:
RAPD is a favorable molecular marker to assist ginseng breeding.
