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Related Experiment Videos

Function of alternative splicing.

Stefan Stamm1, Shani Ben-Ari, Ilona Rafalska

  • 1Institute for Biochemistry, University of Erlangen, Fahrstrasse 17, 91054 Erlangen, Germany. stefan@stamms-lab.net

Gene
|January 20, 2005
PubMed
Summary
This summary is machine-generated.

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Alternative splicing generates diverse mRNA and protein isoforms from limited human genes. This process, alongside nonsense-mediated decay, significantly impacts protein function and abundance, contributing to proteome complexity.

Area of Science:

  • Molecular Biology
  • Genetics
  • Proteomics

Background:

  • Alternative splicing is a key mechanism for generating protein diversity from a limited number of human genes.
  • It alters transcript structure, influencing encoded proteins, unlike promoter activity which mainly affects transcript quantity.
  • Nonsense-mediated decay (NMD) and alternative splicing together are predicted to regulate the abundance of at least 25% of all alternative exons.

Purpose of the Study:

  • To elucidate the functional impact of alternative splicing on protein properties.
  • To understand how alternative splicing factors coordinate biological functions.
  • To highlight alternative splicing as a crucial mechanism for generating proteome complexity in multicellular organisms.

Main Methods:

  • Molecular analyses of alternative splicing events.

Related Experiment Videos

  • Investigation of the effects of alternative splicing on protein binding, localization, activity, stability, and posttranslational modifications.
  • Identification of physiological targets regulated by specific splicing factors.

    • Main Results:

    • Alternative splicing significantly affects protein binding properties, intracellular localization, enzymatic activity, stability, and posttranslational modifications.
    • The impact of alternative splicing ranges from complete loss or gain of function to subtle modulations.
    • Specific splicing factors have been shown to regulate multiple pre-mRNAs with coherent biological functions.

    Conclusions:

    • Alternative splicing is a fundamental process that generates proteome complexity in multicellular organisms.
    • It plays a critical role in coordinating physiologically relevant changes in protein isoform expression.
    • The coordinated regulation of pre-mRNAs by splicing factors underscores the biological significance of alternative splicing.