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Novel mutation method for increased cellulase production.

P Chand1, A Aruna, A M Maqsood

  • 1Department of Microbiology, Osmania University, Hyderabad, India.

Journal of Applied Microbiology
|January 22, 2005
PubMed
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Researchers isolated cellulase-producing fungi and enhanced enzyme production through novel mutation techniques. A new method using sublethal mutagen concentrations yielded potent, stable fungal mutants for increased enzyme output.

Area of Science:

  • Microbiology
  • Biotechnology
  • Enzyme Engineering

Background:

  • Cellulase enzymes are crucial for biomass degradation.
  • Efficient cellulase production is vital for industrial applications.
  • Strain improvement through mutation is a key strategy for enhancing enzyme yields.

Purpose of the Study:

  • To isolate fungi capable of producing cellulase from soil samples.
  • To improve cellulase production in selected fungal strains using novel mutation methods.
  • To identify stable and potent cellulase-producing fungal mutants.

Main Methods:

  • Fungi were isolated using enriched Mandels cellulose agar.
  • Two potent strains were subjected to two mutation methods: direct mutagen treatment and incorporation of mutagens into selective media.

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  • Mutagenesis involved 1-methyl-3-nitro-1-nitrosoguanidine (MNNG), ethidium bromide (EtBr), and UV radiation.

    • Main Results:

    • Seven fungal strains were initially selected, with two identified as potent cellulase producers.
    • Mutation method 2, incorporating mutagens (EtBr, MNNG) in sublethal concentrations into selective media, yielded superior results.
    • This method produced stable, highly potent cellulase-producing mutants, outperforming the first mutation method.

    Conclusions:

    • Employing sublethal mutagen concentrations for extended growth periods effectively generates superior cellulase-producing fungal mutants.
    • This novel mutation approach offers a promising strategy for developing potent fungal strains for enhanced enzyme production.