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From mapping to sequencing, post-sequencing and beyond.

Takuji Sasaki1, Takashi Matsumoto, Baltazar A Antonio

  • 1National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan. tasaki@nias.affrc.go.jp

Plant & Cell Physiology
|January 22, 2005
PubMed
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The Rice Genome Research Program (RGP) established essential tools for rice genome sequencing, contributing significantly to decoding the plant's genetic information. This high-quality genome sequence facilitates advancements in rice breeding and understanding plant biology.

Area of Science:

  • Genomics
  • Plant Biology
  • Agricultural Science

Background:

  • The Rice Genome Research Program (RGP) pioneered large-scale rice genome analysis.
  • Established fundamental research tools: genetic map, physical maps (YAC, PAC/BAC), and transcript map.
  • RGP sequenced six chromosomes, representing 52% of the rice genome.

Purpose of the Study:

  • To elucidate a complete, high-quality sequence of the rice genome.
  • To provide foundational resources for genome research and sequencing.
  • To impact understanding of rice biology and facilitate genomic applications.

Main Methods:

  • Large-scale genome sequencing of rice chromosomes.
  • Development of various mapping resources (genetic, physical, transcript).

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  • Collaboration with the international scientific community.

    • Main Results:

    • Completed a high-quality draft sequence (phase 2) in December 2002.
    • Significant portions of finished quality sequence (phase 3) available in public databases.
    • Full sequencing completion in December 2004.

    Conclusions:

    • The rice genome sequence is crucial for improving existing varieties and developing new ones with enhanced traits.
    • Facilitates innovative research in functional and applied genomics.
    • Generated valuable resources available through the Rice Genome Resource Center (RGRC) for scientific advancement.