Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Monkeypox virus: histologic, immunohistochemical and electron-microscopic findings.

I B Bayer-Garner1

  • 1Marshfield Clinic, Marshfield, WI 54449, USA. bayer-garner.ilene@marshfieldclinic.org

Journal of Cutaneous Pathology
|January 22, 2005
PubMed
Summary

Human monkeypox, a viral zoonosis, presents with distinct skin lesions and fever. Histological examination of biopsies reveals characteristic viral cytopathic effects and inflammation, aiding in diagnosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carcinoma with thymus-like differentiation arising in the dermis of the head and neck.

Journal of cutaneous pathology·2004
Same author

Leukocytoclastic (small vessel) vasculitis in multiple myeloma.

Clinical and experimental dermatology·2003
Same author

Sweet syndrome in multiple myeloma: a series of six cases.

Journal of cutaneous pathology·2003
Same author

AL amyloidosis is not present as an incidental finding in cutaneous biopsies of patients with multiple myeloma.

Clinical and experimental dermatology·2002
Same author

Syndecan-1 (CD138) immunoreactivity in bone marrow biopsies of multiple myeloma: shed syndecan-1 accumulates in fibrotic regions.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2001
Same author

Plasma cells in chronic endometritis are easily identified when stained with syndecan-1.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2001

Area of Science:

  • Pathology
  • Virology
  • Dermatology

Background:

  • Human monkeypox is an emerging viral zoonosis originating in Africa, recently identified in the US.
  • Initial symptoms include skin eruptions, fever, diaphoresis, and rigors.
  • Clinical progression involves lesions evolving from papules to pustules and eschars.

Purpose of the Study:

  • To describe the histological, immunohistochemical, and electron-microscopic features of human monkeypox skin lesions.
  • To aid in the differential diagnosis of monkeypox from other viral exanthems.

Main Methods:

  • Analysis of three cutaneous biopsy specimens from two patients with PCR-proven monkeypox.
  • Utilized histology, immunohistochemistry, and electron microscopy.

Related Experiment Videos

Main Results:

  • Histological findings mirror clinical progression, showing basal keratinocyte degeneration, spongiosis, and epidermal necrosis.
  • Inflammation involves epidermis, vascular plexuses, eccrine units, and follicles.
  • Viral cytopathic effects include multinucleated syncytial keratinocytes; viral antigen detected in epidermal and follicular cells.

Conclusions:

  • Histological differential diagnosis includes herpes simplex virus, varicella, and smallpox.
  • Herpes simplex virus can be differentiated by all methods.
  • Varicella and smallpox are best differentiated by PCR assay for the monkeypox virus gene.