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Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
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IFN-gamma overcomes low responsiveness of myeloid dendritic cells to CpG DNA

Masato Uchijima1, Toshi Nagata, Taiki Aoshi

  • 1Department of Microbiology and Immunology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Immunology and Cell Biology
|January 22, 2005
PubMed

Dendritic cells (DC) are professional APC that have an extraordinary capacity to prime naive T cells. It has been reported that human DC subsets express distinct toll-like receptor (TLR), which influences their function. In mice, we observed that plasmocytoid DC (pDC) express a higher level of TLR9 compared with myeloid DC (mDC) cultured with GM-CSF. However, we demonstrated that stimulation with IFN-gamma is capable of upregulating TLR9 expression in mDC to a level comparable with expression in pDC. Consistent with this observation, IL-12 p40 and IL-6 mRNA expression and IL-12 p70 secretion in response to CpG-oligodeoxynucleotides are enhanced in mDC pretreated with IFN-gamma compared with untreated cells. Therefore, TLR-mediated responses of DC subsets may be influenced not only by signals delivered by pathogens but also by regulatory signals from cytokines such as IFN-gamma.

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