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Updated: Aug 20, 2026

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
Published on: August 23, 2024
Sonochemical reduction of the antioxidant activity of olive mill wastewater
Dora Atanassova1, Panagiotis Kefalas, Christos Petrakis
1Laboratory of Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania, PO Box 85, Chania GR-73100, Greece.
Abstract:
The use of ultrasonic irradiation to reduce the antioxidant activity of olive oil mill wastewaters (OMWs) originating from two-phase and three-phase decanters was examined. Sonication of diluted OMW samples was conducted at ultrasonic frequencies of 24 and 80 kHz, an applied power varying between 75 and 150 W, and liquid bulk temperatures varying between 25 and 60 degrees C. At the conditions in question, the reduction in antioxidant activity was found to increase with decreasing temperature and increasing power and frequency. Addition of NaCl in the samples also appeared to enhance reduction. Antioxidant activity of OMW samples was assessed using the recently developed Co(II)/ethylenediaminetetraacetic acid (EDTA)-induced luminol chemiluminescence analytical protocol, while the total phenolic load was measured according to the Folin-Ciocalteu method.
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