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Related Experiment Videos

High-performance liquid chromatography method for the quantification of rabeprazole in human plasma using solid-phase

N V S Ramakrishna1, K N Vishwottam, S Wishu

  • 1Biopharmaceutical Research, Suven Life Sciences Ltd., Serene Chambers, Road #7, Banjara Hills, Hyderabad 500034, India. nvsrk@suven.com

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|January 25, 2005
PubMed
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A new HPLC method accurately quantifies rabeprazole (a proton-pump inhibitor) in human plasma. This validated assay is sensitive and repeatable for pharmacokinetic studies.

Area of Science:

  • Analytical Chemistry
  • Pharmacology

Background:

  • Rabeprazole is a new proton-pump inhibitor.
  • Accurate quantification in human plasma is crucial for pharmacokinetic studies.

Purpose of the Study:

  • To develop and validate a simple, sensitive, and selective High-Performance Liquid Chromatography (HPLC) method for rabeprazole quantification in human plasma.

Main Methods:

  • Solid-phase extraction (SPE) using Waters Oasis cartridges.
  • Isocratic mobile phase (ammonium acetate buffer/acetonitrile/methanol) on a Waters symmetry C18 column.
  • UV detection at 284 nm.

Main Results:

  • Lower limit of quantitation (LLOQ): 20 ng/mL with <8% relative standard deviation.
  • Linear range: 20-1000 ng/mL.

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  • Excellent method validation: within-batch precision (2.2-7.3%), between-batch precision (2.4-7.2%), and bias (-2.6% to 2.6%).
  • No interference from coadministered drugs.
  • Rabeprazole stability confirmed during processing and 3-month storage.

    • Conclusions:

    • The developed HPLC method is sensitive, simple, and repeatable.
    • The method is suitable for pharmacokinetic studies of rabeprazole.