Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MSP dynamics drives nematode sperm locomotion.

Charles W Wolgemuth1, Long Miao, Orion Vanderlinde

  • 1University of Connecticut Health Center, Department of Cell Biology, Farmington, Connecticut 06030-3505, USA.

Biophysical Journal
|January 25, 2005
PubMed
Summary

Cell crawling relies on cytoskeletal dynamics. This study models how major sperm protein disassembly generates retraction forces, explaining forward cell propulsion in Ascaris suum.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IGF2BP3 mediates the mRNA upregulation of NLRP3 to promote parathyroid cell proliferation in chronic kidney disease via an m⁶A-dependent manner.

Endocrine·2026
Same author

CRISPR/Cas9-mediated Endogenous Fluorescent Tagging of Germline-specific Genes in Caenorhabditis elegans.

Journal of visualized experiments : JoVE·2026
Same author

Childhood violence across distinct, overlapping, and concurrent contexts: polyvictimization, polyperpetration, and missed interventions points among child knife crime fatalities in England.

Frontiers in sociology·2026
Same author

Natural variation of the <i>GmDt1</i> gene affects the 100-seed weight of soybean.

Frontiers in plant science·2026
Same author

Preinjury, injury and post-injury factors leading to death in children and young people who were victims of knife crime in England between 2019 and 2024: a review of the National Child Mortality Database.

Emergency medicine journal : EMJ·2026
Same author

Bronchial Compression Following Surgical and Transcatheter Interventions for Coarctation of the Aorta.

Journal of the Society for Cardiovascular Angiography & Interventions·2026

Area of Science:

  • Cell biology
  • Biophysics
  • Biochemistry

Background:

  • Eukaryotic cell motility is crucial for biological processes.
  • Cell crawling typically involves polymerization, adhesion, and rear retraction.
  • The forces driving retraction are not fully understood.

Purpose of the Study:

  • To investigate the mechanism of retraction forces in cell crawling.
  • To model disassembly-induced retraction using major sperm protein (MSP) cytoskeleton.
  • To explain how cytoskeleton disassembly generates force for cell body propulsion.

Main Methods:

  • In vitro experiments using extracts from Ascaris suum spermatozoa.
  • Development of a kinetic model for MSP cytoskeleton disassembly.
  • Integration of polymer entropy into the disassembly model.

Related Experiment Videos

Main Results:

  • MSP cytoskeleton depolymerization generates retraction forces.
  • The proposed model accurately fits in vitro experimental data.
  • The model elucidates the mechanism of disassembly-induced retraction.

Conclusions:

  • Cytoskeleton disassembly is a key mechanism for generating retraction forces in cell crawling.
  • The model provides a quantitative explanation for force generation during cell motility.
  • Further experiments are suggested to validate the model's predictions.