Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glycolytic enzymes can modulate cellular life span.

Hiroshi Kondoh1, Matilde E Lleonart, Jesus Gil

  • 1Wolfson Institute for Biomedical Research, University College London, London Research Institute, London, United Kingdom. hiroshi.kondoh@cancer.org.uk

Cancer Research
|January 25, 2005
PubMed
Summary

Researchers discovered that the glycolytic enzyme phosphoglycerate mutase (PGM) can immortalize cells. Increased PGM activity promotes cell proliferation and resistance to arrest, while its depletion causes senescence.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine Learning-Assisted Development of High-Performance Ethanol Synthesis Catalysts via CO<sub>2</sub> Hydrogenation.

Journal of the American Chemical Society·2026
Same author

Severe OSA May Be Associated With Endothelial Dysfunction in Patients With Nocturnal Hypertension.

Archivos de bronconeumologia·2026
Same author

Tumoral switch in NUMB splicing changes essential transcription pathways and induces malignant properties in tumour cells.

Genome biology·2026
Same author

Role of WDR66 in Stemness, Therapy Resistance and Tumor microenvironment modulation in Head and Neck Cancer.

International journal of biological sciences·2026
Same author

The Warburg effect provides attributes that facilitate tumor-cell resistance.

Histology and histopathology·2026
Same author

Artificial Intelligence in Tumor Evolution: Understanding Cancer Complexity Through Multi-Modal Data Integration in Precision Oncology.

Cells·2026

Area of Science:

  • Cell Biology
  • Biochemistry
  • Cancer Research

Background:

  • Cellular senescence is a state of irreversible growth arrest.
  • Understanding the molecular mechanisms of cellular immortalization is crucial for cancer research.

Purpose of the Study:

  • To identify genes involved in cellular immortalization.
  • To investigate the role of glycolysis in cell proliferation and senescence.

Main Methods:

  • Unbiased genetic screening in mouse embryonic fibroblasts.
  • Short interfering RNA (siRNA) for gene depletion.
  • Assays for glycolytic flux, proliferation, and senescence.

Main Results:

  • Phosphoglycerate mutase (PGM), a glycolytic enzyme, was identified as a gene that can immortalize cells.

Related Experiment Videos

  • Increased PGM activity enhanced glycolytic flux, enabled indefinite proliferation, and conferred resistance to ras-induced arrest.
  • Depletion of PGM or glucosephosphate isomerase using siRNA triggered premature senescence.
  • Immortalized cells and embryonic stem cells showed higher glycolytic flux and oxidative damage resistance compared to senescent cells.
  • Wild-type p53 down-regulates PGM, suggesting p53 mutations facilitate immortalization by affecting PGM levels and glycolysis.

    • Conclusions:

    • Glycolytic enzymes, specifically PGM, play a significant role in cellular immortalization.
    • Modulating glycolytic flux is a potential strategy for controlling cell proliferation and senescence.
    • The p53 pathway interacts with glycolysis to regulate cell fate decisions, offering insights into cancer development.