Related Experiment Video
Updated: Aug 19, 2026

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
Published on: June 16, 2011
Amyloid-beta induced cell death is independent of free radicals
Alexander Boldyrev1, Alexey Koudinov, Temirboulat Berezov
1International Biotechnological Center, M.V. Lomonosov Moscow State University, Department of Biochemistry, 119992 Moscow, Russia. aaboldyrev@mail.ru
Abstract:
Acutely dissociated rat cerebellar granule cell neurons were incubated with amyloid-beta (1-42) and studied by flow cytometry. Amyloid-beta caused a dose-dependent loss of viability, as determined by intracellular accumulation of propidium iodide (PI),and that was not accompanied by significant elevation of intracellular calcium, measured by Fluo-3 or reactive oxygen species (ROS), measured by 2',7'-dihydro-dichlorfluorescein diacetate (DCF). Carnosine, a ROS scavenger and an inhibitor of non-enzymatic glycosylation, partially reduced cell death caused by amyloid-beta. We conclude that amyloid-beta causes a relatively acute loss of cell viability in cerebellar granule cell neurons, which does not result from either elevation of intracellular calcium concentration or generation of ROS.
Related Concept Videos
Overview of Cell Death
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
Cellular Injury IV: Necrosis
Cellular Injury I: Introduction
Bioactivation and Tissue Toxicity
Cellular Injury V: Apoptosis and Autophagy
The Extrinsic Apoptotic Pathway

