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Isolation of Mouse Peritoneal Cavity Cells
04:32

Isolation of Mouse Peritoneal Cavity Cells

Published on: January 28, 2010

B1-B cells are the main antigen presenting cells in CpG-ODN-stimulated peritoneal exudate cells

Hiromichi Bamba1, Hirohito Ishigaki, Hideaki Ishida

  • 1Department of Pathology, School of Medicine, Shiga University of Medical Science, Ohtsu, Shiga, Japan.

Microbiology and Immunology
|January 25, 2005
PubMed

Peritoneal exudate cells (PEC) have long been used as antigen presenting cells (APC), because they have been considered to contain mainly macrophages. However, it is still unclear specifically which cells of the peritoneal exudate function as APC. Herein, we focused on macrophages and B1-B cells of the PEC and examined their APC function and cytokine production. B1-B cells purified from PEC functioned effectively as APC after CpG-stimulation and mainly produced IL-10. In contrast, macrophages purified from PEC were not able to present incorporated antigens to T cells, despite the production of IL-12 and expression of co-stimulatory molecules after CpG stimulation. These results suggest that previously held ideas regarding the functions of the mixture of cells in the PEC need to re-evaluated. In summary, the antigen presenting function of PEC was mainly attributed to B1-B cells and immunoenhancing cytokine production was dominantly derived from peritoneal macrophages.

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