Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple negative feedbacks on CD152 expression in allograft tolerance.

Meng-Kun Tsai1, Hong-Nerng Ho, Hsiung-Fei Chien

  • 1Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Chung-Shan South Road,Taipei, 100, Taiwan.

Transplantation
|January 25, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ascitic Fluid Drainage Before Dialysis Catheter Removal for Peritoneal Dialysis-Related Peritonitis.

Kidney & blood pressure research·2026
Same author

Conserved enhancer association of piRNAs and the implication in germ cell fate surveillance.

Genome biology·2026
Same author

ZNRF1 deficiency disrupts Fas ligand trafficking and immune balance.

Cell death & disease·2026
Same author

Posttransplant screening and short-course treatment of latent tuberculosis infection in kidney transplant recipients: A prospective observational study in Taiwan.

Pulmonology·2026
Same author

Pathophysiology of androgen-associated endothelial cell dysfunction in phenotype A polycystic ovarian syndrome revealed by iPSCs modeling.

Frontiers in endocrinology·2026
Same author

Modulating metabolic signatures to mitigate cabozantinib resistance in FLT3-ITD acute myeloid leukemia cell models.

Cell death discovery·2026

CD80 and CD86 regulate immune tolerance by controlling CD152 expression. This study reveals how these molecules impact T-cell proliferation and allograft acceptance in a cardiac transplant model.

Area of Science:

  • Immunology
  • Transplantation immunology
  • Molecular biology

Background:

  • Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4, or CD152) is crucial for immune tolerance.
  • The precise mechanisms by which CD80 and CD86 regulate CD152 expression remain incompletely understood, particularly in the context of long-term allograft acceptance.

Purpose of the Study:

  • To investigate the regulatory roles of CD80 and CD86 in modulating CD152 expression and function.
  • To elucidate the molecular mechanisms underlying CD152-mediated allograft tolerance in a cardiac transplant model.

Main Methods:

  • Utilized a low-responding BALB/c to B10.A cardiac transplant model.
  • Employed donor-specific stimulation and antibody blockade of the CD80/CD86:CD28/CD152 pathway in recipient splenic T cells.

Related Experiment Videos

  • Conducted proliferation assays, quantitative PCR, flow cytometry, and fluorescence microscopy.

    • Main Results:

    • T cells from recipients with long-term grafts (B10.A-100) exhibited hyporesponsiveness.
    • Blockade of CD80, CD86, or CD152 enhanced T-cell proliferation.
    • CD152 inhibited T-cell proliferation by reducing CD152 expression, mediated by CD80 and CD86 interactions.
    • CD28 engagement promoted intracellular CD152 accumulation, while CD80 engagement decreased CD152 mRNA, and CD86 engagement inhibited surface CD152 expression.

    Conclusions:

    • CD80 and CD86 exert multiple negative feedback controls on both CD152 mRNA transcription and surface expression.
    • These regulatory interactions are critical for establishing and maintaining CD152-mediated allograft tolerance.