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An improved micromethod for tyrosine estimation.

Munjal M Acharya1, Surendra S Katyare

  • 1Department of Biochemistry, Faculty of Science, M.S. University of Baroda, Sayajigunj, Vadodara 390002, Gujarat, India. munjalres@yahoo.com

Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|January 26, 2005
PubMed
Summary

A new, improved micromethod for tyrosine determination offers enhanced sensitivity and economic efficiency. This method is suitable for enzymatic reactions and tissue analysis, providing reliable results comparable to conventional procedures.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Accurate tyrosine determination is crucial for various biochemical assays.
  • Existing methods may lack sensitivity or be resource-intensive.

Purpose of the Study:

  • To develop a modified and improved micromethod for tyrosine determination.
  • To enhance sensitivity, economic efficiency, and applicability in biochemical analyses.

Main Methods:

  • Optimization of Folin-Ciocalteu (FC) reagent concentration and conditions.
  • Colorimetric analysis at 750 nm for tyrosine quantification.
  • Validation in enzymatic reactions (lysosomal cathepsin D) and tissue samples.

Main Results:

  • The optimized micromethod uses 0.2 N FC reagent for rapid color development.

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  • Linearity observed for 2-16 microg tyrosine, offering a 3-fold increase in sensitivity.
  • Stable blue color formation for up to 24 hours.
  • Identical results obtained compared to conventional methods in enzymatic and tissue assays.

    • Conclusions:

    • The developed micromethod is sensitive, economical, and rapid for tyrosine determination.
    • It is applicable for routine biochemical analysis, including enzymatic assays and tissue studies.
    • The method provides a reliable and efficient alternative to conventional procedures.