Related Experiment Video
Updated: Aug 19, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 12, 2014
Quantitative DNA measurement in oropharyngeal squamous cell carcinoma and surrounding mucosa
S F Preuss1, J Brieger, E K Essig
1Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital, School of Medicine, Mainz, Germany.
Objective:
The appearance of local recurrences, distant metastases and second primary tumors limit the survival rate of patients with head and neck squamous cell carcinomas. The presence of genetic alterations at the resection margins is an indicator of inadequate excision and a predictor of local failure.
Design:
To receive insight into the genetic abnormalities in the mucosa surrounding oropharyngeal cancers, we performed a quantitative DNA measurement in the primary tumor and the surrounding areas.
Results:
We found a high level of DNA irregularity in primary tumors. The DNA irregularity decreased in specimens taken at a distance of 1 and 2 cm from the tumor margin.
Conclusions:
The genetically altered field is thought to determine the risk for recurrence of a second tumor. We therefore believe that the genetic analysis of tissue surrounding the tumor could become a feasible diagnostic procedure to determine the individual risk for local recurrence or second primary tumors.
More Related Videos
07:43Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Published on: July 29, 2017
06:57Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
Published on: June 14, 2019