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Related Experiment Videos

Insect-resistant transgenic Pinus radiata.

Lynette J Grace1, Julia A Charity, Belinda Gresham

  • 1New Zealand Forest Research Institute Ltd, Private Bag 3020, Rotorua, New Zealand. lynette.grace@forestresearch.co.nz

Plant Cell Reports
|January 26, 2005
PubMed
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Transgenic radiata pine engineered with a Bacillus thuringiensis (Bt) toxin gene show promising insect resistance. One line demonstrated high resistance against the painted apple moth, indicating potential for pest management in forestry.

Area of Science:

  • Plant Biotechnology
  • Forest Genetics
  • Molecular Entomology

Background:

  • Radiata pine (Pinus radiata D. Don) is a commercially important timber species.
  • Insect pests pose a significant threat to pine plantations, impacting yield and health.
  • Genetic modification offers a potential strategy for enhancing tree resilience to pests.

Purpose of the Study:

  • To develop transgenic radiata pine expressing the Bacillus thuringiensis (Bt) crylAc gene.
  • To assess the integration, expression, and insecticidal efficacy of the introduced Bt gene.
  • To evaluate the potential of genetically modified pine for insect pest resistance.

Main Methods:

  • Biolistic transformation of embryogenic radiata pine tissue with the crylAc gene.
  • Selection of transgenic events using the nptII marker gene and geneticin.

Related Experiment Videos

  • Confirmation of gene integration and expression via PCR, Southern hybridization, NPTII, and Bt ELISA assays.
  • Bioassays using painted apple moth (Teia anartoides) larvae to assess insect resistance.

    • Main Results:

    • Successfully generated 20 independent transgenic radiata pine lines across five genotypes.
    • Confirmed stable integration and expression of the Bt crylAc gene in transgenic plants.
    • Demonstrated variable levels of insect resistance in transgenic lines, with one line showing high resistance to Teia anartoides feeding damage.

    Conclusions:

    • Transgenic radiata pine expressing the Bt crylAc gene can be produced through biolistic transformation.
    • The developed transgenic lines exhibit varying degrees of resistance to insect herbivory.
    • This research provides a foundation for developing insect-resistant forest trees, potentially reducing reliance on chemical pesticides.