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A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
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New approaches to the formation of a stationary phase in an immunoadsorption wall

Tsung-Hua Yang1

  • 1Department of Chemical Engineering, Cheng Shiu University, Kaohsiung, Taiwan, Republic of China. thyang@csu.edu.tw

Artificial Organs
|January 27, 2005
PubMed

The concept of an immunoadsorption wall, which combines the principles of immunoisolation and immunoadsorption, was proposed in 1999 to remove certain toxins accumulated in patients' blood. However, realization of this concept is obviously handicapped by the inefficient use of immunoadsorbent. This study is intended to improve the use of immunoadsorbent and optimize the formation of a stationary phase in an immunoadsorption wall. Polyacrylamide gel, which has the advantages of being chemically inert, having minimal diffusion effect and reasonable cost, could be considered as the medium of choice for a stationary phase. In this study, new approaches aimed at effective allocation of immunoadsorbent utilizing polyacrylamide gel are attempted. The advantages and disadvantages of these new approaches are discussed according to the preparation, formation, and outcome of a stationary phase. It is hoped that these new approaches could serve as a first step toward building an immunoadsorption wall.

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