Related Experiment Video
Updated: Aug 19, 2026

Seven Steps to Stellate Cells
Published on: May 10, 2011
[Regulation of hepatic stellate cell activation by interleukin-10/platelet derived growth factor/mitogen-activated
Tao Li1, Xi-Sheng Leng, Zhi-Zhong Qin
1Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing 100044, China.
Objective:
To investigate the regulatory effect of interleukin-10 (IL10) on the activation of hepatic stellate cells (HSC) through platelet derived growth factor (PDGF) and mitogen-activated protein kinase (MAPK) pathways.
Methods:
HSC were divided randomly into 4 groups. Group 1 served as a control. HSC were incubated with 1 ng/ml, 5 ng/ml, and 25 ng/ml IL-10 in groups 2, 3 and 4. RT-PCR and western blot were used to detect the expression of PDGF and MAPK protein ERK and p38 and alpha-SMA.
Results:
Compared with the control group, expressions of ERK, p38 and alpha-SMA of groups 2, 3 and 4 were significantly lower (F values were 240.47, 21.39, 28.86 respectively. IL-10 inhibited PDGF and MAPK protein ERK and p38 and alpha-SMA expression in a dose-dependent way.
Conclusion:
IL-10 inhibits activation of HSC through the PDGF/MAPK pathway.
Related Concept Videos
Liver Regeneration
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
The JAK-STAT Signaling Pathway
Cirrhosis II: Pathophysiology
PI3K/mTOR/AKT Signaling Pathway
Regulation of Hematopoietic Stem Cells
Regulation of Angiogenesis and Blood Supply
